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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C14095-100 100 µg $665 
Antibody

Monoclonal Mouse anti‑Human ITGB4 / Integrin Beta 4 Antibody (IHC) LS‑C14095

Monoclonal Mouse anti‑Human ITGB4 / Integrin Beta 4 Antibody (IHC) LS‑C14095

Antibody:
Mouse Monoclonal to Human ITGB4 / Integrin Beta 4
Application:
IHC, IHC-Fr, IP, Flo, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$665 
LS-C14095-100
100 µg
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Mouse Monoclonal to Human ITGB4 / Integrin Beta 4
Application:
IHC, IHC-Fr, IP, Flo, Func
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Integrin Beta 4 antibody LS-C14095 is an unconjugated mouse monoclonal antibody to human Integrin Beta 4 (ITGB4). Validated for Flow, Func, IHC and IP.
Target
Human ITGB4 / Integrin Beta 4
Synonyms
ITGB4 | CD104 | Integrin beta-4 | Integrin Beta 4 | gp150 | CD104 antigen | Integrin beta-4 subunit | Integrin, beta 4
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Human squamous cell carcinoma cells (SCC9).
Specificity
Human b4 integrin (CD104).
Usage
Inhibition of cell attachment: inhibits SCC9 cell adhesion to human and EHS laminin, but not to laminin GD-2 peptide, type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen. Immunohistochemistry: on acetone-fixed frozen human epithelial tissues including tongue, foreskin and ovary. Not for use on formalin-fixed tissue. Flow cytometry: stains human squamous cell carcinoma (SCC9), ovarian carcinoma (SKOV-3) and umbilical vein endothelium (HUVEC). Stains RKO or K562 cells transfected for CD104 expression. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets. Immunoprecipitation: precipitates PAGE bands of 66, 76, 120, 135 and 200kD (reduced) or 66, 110, 125, 130 and 190kD (non-reduced) from surface-biotinylated SCC9 cells.
Presentation
0.02 M PB, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGB4 / Integrin Beta 4
P16144 NM_000213 NP_000204.3

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Requested From: United States
Date Requested: 7/9/2020