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Catalog Number Size Price
LS-C45336-100 100 Tests $456 
Antibody

Monoclonal Rat anti‑Mouse ITGAM / CD11b Antibody (clone M1/70.15, RPE) LS‑C45336

Monoclonal Rat anti‑Mouse ITGAM / CD11b Antibody (clone M1/70.15, RPE) LS‑C45336

Note: This antibody replaces LS-C58658
Antibody:
ITGAM / CD11b Rat anti-Mouse Monoclonal (RPE) (M1/70.15) Antibody
Application:
Flo
Reactivity:
Mouse
Format:
R. Phycoerythrin, Unmodified
Price
Catalog Number
$456
LS-C45336-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ITGAM / CD11b Rat anti-Mouse Monoclonal (RPE) (M1/70.15) Antibody
Application:
Flo
Reactivity:
Mouse
Format:
R. Phycoerythrin, Unmodified

Specifications

Description
CD11b antibody LS-C45336 is an RPE-conjugated rat monoclonal antibody to mouse CD11b (ITGAM). Validated for Flow.
Target
Mouse ITGAM / CD11b
Synonyms
ITGAM | Antigen CD11b (p170) | CD11B | CD11b antigen | CR3A | Integrin alpha-M | MAC-1 | MO1A | Neutrophil adherence receptor | MAC1A | SLEB6 | CR-3 alpha chain
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
M1/70.15
Conjugations
R. Phycoerythrin
Purification
Protein G purified
Modifications
Unmodified
Immunogen
T cells enriched splenocytes from B10 mice.
Specificity
Recognizes the murine CD11b cell surface antigen (also known as the alpha M integrin chain or MAC-1), a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Clone M1/70.15 has been reported to block iC3b binding to its receptor. * Clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue. This product is routinely tested in flow cytometry on mouse peritoneal macrophages.
Applications
  • Flow Cytometry (1:1 - 1:10)
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.09% Sodium Azide, 5% Sucrose, 1% BSA
Reconstitution
Reconstitute with distilled water.
Storage
Store at 4°C. Do not freeze. Store undiluted. Protect from light. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ITGAM / CD11b
P11215 NM_000632 NP_000623.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/18/2022