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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRS1 Antibody (aa837‑1089, Biotin) LS‑C687212
IRS1 antibody LS-C687212 is a biotin-conjugated rabbit polyclonal antibody to mouse IRS1. Validated for IHC and WB.
Catalog
Size
Price
LS-C687212-20
20 µl
$205
LS-C687212-100
100 µl
$275
LS-C687212-200
200 µl
$315
LS-C687212-1
1 ml
$590
LS-C687212-10
10 ml
$2,370

Popular IRS1 Products

Anti-IRS1 antibody IHC of human brain. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1373 concentration 5 ug/ml.
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Antibody
Species: Mouse, Human, Rat
Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation
Antibody
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig
Applications: ICC, Western blot, Immunoprecipitation
IHC of p-IRS-1 (S636) pAb in paraffin-embedded human brain tissue.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-IRS1 pS307 Antibody - Western Blot. Western blot of Affinity Purified anti-IRS1 pS307 antibody shows detection of a band at ~180 kD believed to represent phosphorylated IRS1 (arrowhead). Lane 1 shows staining of human 293 cell lysate. Lane 2 shows staining of 293 cell lysate prepared from cells serum-starved for 18 h followed by treatment with 5 ug/ml of anisomysin for 30 min. The pronounced staining of the band at 180 kD is not seen when the antibody was pre-incubated with immunizing peptide prior to reaction (data not shown). The identity of the intensely reactive bands at ~70 kD in both lane 1 and 2 is unknown, although these bands were also competed out by pre-incubation with the immunizing peptide. Approximately 25 ug of each lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% goat serum, 0.5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1:250. Reaction occurred overnight at 4C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Species: Human
Applications: Western blot, ELISA

Product Description

IRS1 antibody LS-C687212 is a biotin-conjugated rabbit polyclonal antibody to mouse IRS1. Validated for IHC and WB.
About IRS1
May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. P35568 NM_005544 NP_005535.1

IRS1 Antibody, IRS-1 Antibody, HIRS-1 Antibody, Insulin receptor substrate 1 Antibody


Specifications

Target
Mouse IRS1
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin. Also available Unconjugated or conjugated with Biotin, FITC, Cy3, HRP, APC, PE, APC, Cy7.
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IRS1 antibody was raised against recombinant IRS1 (Pro837-Gln1089) expressed in E. coli.
Epitope
aa837-1089
Specificity
The antibody is a rabbit polyclonal antibody raised against IRS1. It has been selected for its ability to recognize IRS1 in immunohistochemical staining and western blotting.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Avoid repeated freeze/thaw cycles. Store at 4°C for frequent use. Aliquot and store at -20°C for 12 months.
Restrictions
For research use only.

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Images

Western blot

Western blot of recombinant IRS1 / IRS-1.
Western blot of recombinant IRS1 / IRS-1.

Western blot

Western blot of recombinant IRS1 / IRS-1.
Western blot of recombinant IRS1 / IRS-1.

Western blot

Western blot of recombinant IRS1 / IRS-1.
Western blot of recombinant IRS1 / IRS-1.

Western blot

Western blot of recombinant IRS1 / IRS-1.
Western blot of recombinant IRS1 / IRS-1.

Requested From: United States
Date Requested: 9/26/2018

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