IRS1 antibody LS-C16084 is an unconjugated rabbit polyclonal antibody to IRS1 from human, mouse and rat. Validated for IP and WB.
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot (0.5 - 2 µg/ml)
- (applications tested for the base form of this product only)
IRS1 antibody was raised against precarboxy-terminal peptide (16aa) corresponding to aa1220-1235 of rat liver IRS-1 (KLH coupled).
Recognizes rat IRS-1, Mr 160-185kD. Does not recognize IRS-2. Species cross-reactivity: Human and mouse.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 0.5-2 ug/ml detected IRS-1 in a 3T3/A31 RIPA cell lysate. Immunoprecipitation: 4 ug immunoprecipitated IRS-1 from 500 ug of a 3T3/A31 RIPA lysate.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.