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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF5 Antibody (aa81‑108) LS‑C97318
IRF5 antibody LS-C97318 is an unconjugated rabbit polyclonal antibody to human IRF5. Validated for Flow, IF and WB.
Catalog
Size
Price
LS-C97318-400
400 µl
$305

Popular IRF5 Products

Immunoperoxidase of monoclonal antibody to IRF5 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.8 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
IRF5 antibody Western blot of SH-SYSY lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit, Zebrafish
Applications: Western blot
Immunoperoxidase of monoclonal antibody to IRF5 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1.5 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Antibody
Species: Mouse, Rat
Applications: Western blot, Immunoprecipitation, ELISA

Product Description

IRF5 antibody LS-C97318 is an unconjugated rabbit polyclonal antibody to human IRF5. Validated for Flow, IF and WB.

Specifications

Target
Human IRF5
Synonyms
IRF5, Interferon regulatory factor 5, IRF-5, SLEB10, IBD14
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Blocking Peptide
LS-E7128 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C97318. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa81-108
Specificity
This IRF5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human IRF5.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
About IRF5
IRF5 is a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Q13568 NM_002200 NP_116032.1


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Images

Immunofluorescence

Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.
Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.
Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.
Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence

Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Confocal immunofluorescence of IRF5 Antibody with A549 cell followed by Alexa Fluor 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot

Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.
Western blot of anti-IRF5 Antibody in Ramos cell line lysates (35 ug/lane). IRF5 (arrow) was detected using the purified antibody.

Flow Cytometry

IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
IRF5 Antibody flow cytometry of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 11/12/2018

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