(tested or 100% immunogen sequence identity)
IRF5 antibody was raised against recombinant human IRF5 (176-240aa) purified from E. coli.
Recognizes human interferon regulatory factor 5 (IRF5), a 58kD member of the IRF family which acts as a key regulator of interferon gene transcription, and is expressed in B cells and dendritic cells, and is inducible by type I interferon (IFN) in most lymphoid cells. The role of IRF3 and IRF7 as transcriptional mediators of virus- and IFN-induced signaling pathways is well documented. Studies have shown that IRF5 can also regulate IFN gene transcription, but appears to be more restricted in its cellular expression and range of viral stimuli, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10.
Western Blot: LS-C45031 detects a band of approximately 58kD in THP-1 cell lysates.
PBS, pH 7.4, 0.02% Thimerosal