(tested or 100% immunogen sequence identity)
Immunohistochemistry: LS-B182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B182 was determined to be 5 ug/ml.
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.