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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF3 Antibody (Internal) IHC‑plus™ LS‑B182 Best Seller
Note: This antibody replaces LS-C457, LS-C56876
IRF3 antibody LS-B182 is an unconjugated rabbit polyclonal antibody to human IRF3. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B182-50
50 µg (1 mg/ml)
Unavailable

Popular IRF3 Products

Antibody
Species: Human
Applications: Western blot, ELISA
Anti-IRF3 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2529 dilution 1:50.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin, Western blot
Immunofluorescent staining using IRF3 antibody. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-IRF3 antibody.
Species: Human
Applications: ICC, Western blot, Immunoprecipitation, Flow Cytometry, Dot Blot
Anti-IRF3 Antibody (phospho-Ser396) antibody at 1:500 dilution with Jurkat cells treated with 200 ng/ml of EGF for 30 minutes.
Species: Human, Mouse, Rat
Applications: Western blot, ELISA

Product Description

IRF3 antibody LS-B182 is an unconjugated rabbit polyclonal antibody to human IRF3. Validated for ICC, IHC and WB. Tested on 20 paraffin-embedded human tissues.
About IRF3
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Q14653 NM_001571 NP_001562.1

IRF3 Antibody, Interferon regulatory factor 3 Antibody, IRF-3 Antibody


Specifications

Target
Human IRF3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Western blot (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E30094 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B182. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Internal
Usage
Immunohistochemistry: LS-B182 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B182 was determined to be 5 ug/ml.
Presentation
PBS, 0.02% sodium azide.
Storage
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.
Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Western blot

Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..

Western blot

Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunohistochemistry

Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.
Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Western blot

Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..

Western blot

Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunohistochemistry

Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.
Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Western blot

Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..

Western blot

Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

Immunohistochemistry

Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.
Anti-IRF3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B182 concentration 5 ug/ml.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunocytochemistry

Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunocytochemistry of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 µg/mL.

Immunofluorescence

Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.
Immunofluorescence of IRF3 in Ramos cells with IRF3 antibody at 2 ug/ml.

Western blot

Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..
Western blot analysis of IRF3 in (A) human kidney and (B) rat kidney lysate with IRF3 antibody at 1 µg/mL..

Western blot

Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.
Western blot of IRF3 in U937 whole cell lysate with IRF3 antibody at (A) 1, (B) 2, and (C) 4 ug/ml.

Requested From: 
Date Requested: 7/19/2018

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