IRF3 antibody LS-B135 is an unconjugated rabbit polyclonal antibody to IRF3 from human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (5 - 10 µg/ml)
- Immunofluorescence (20 µg/ml)
- Western blot (1 - 2 µg/ml)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
- Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-B135. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
At least three isoforms of IRF3 are known to exist.
Immunohistochemistry: LS-B135 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B135 was determined to be 5-10 ug/ml.
PBS, 0.02% Sodium Azide
Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.
For research use only.