(tested or 100% immunogen sequence identity)
IRF3 antibody was raised against e. coli-derived rhIRF3 (aa206-427).
Selected for its ability to recognize human IRF3 in the applications listed below. In direct ELISA and Western Blots, this antibody shows ~60% cross-reactivity with rmIRF3.
Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Western Blot: 1 ug/ml (w/ appropriate secondary reagents). Detection limit is ~10 ng/lane (non-reducing) and ~1 ng/lane (reducing). Flow Cytometry: 50 ug/ml and add 10 ul of the diluted solution to 1-2.5x10^5 HeLa cells in a total reaction volume 200ul. The binding of unlabeled antibodies may be visualized by adding 10 ul of a 25 ug/ml stock solution of a secondary developing reagent such as anti-goat IgG (FITC). Immunocytochemistry: 10 ug/ml detects human IRF3 in HeLa cells. Cells were fixed with PBS, 4% paraformaldehyde for 20 min at RT and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 min at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS, 0.1% BSA. ELISA: 0.5-1.0 ug/ml (w/ appropriate secondary reagents), detection limit is ~0.2 ng/well.
100 µl 40-50% glycerol, PBS.
Store at 4°C or -20°C. Avoid freeze-thaw cycles.