IRF3 antibody LS-C45030 is an unconjugated mouse monoclonal antibody to human IRF3. Validated for ELISA and WB.
(tested or 100% immunogen sequence identity)
Protein G purified
- Western blot (1:2000 - 1:3000)
IRF3 antibody was raised against recombinant human IRF3 (108-166aa) purified from E. coli.
Recognizes human interferon regulatory factor 3 (IRF3), a 55kD constitutively expressed member of the IRF family, which acts as vital regulator of gene expression in innate immunity. IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta, along with other interferon-induced genes.
Western Blot: LS-C45030 detects a band of approximately 55kD in HeLa cell lysates.
PBS, pH 7.4, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.