(tested or 100% immunogen sequence identity)
- IHC (1:50 - 1:200)
- IHC - Paraffin
- Immunofluorescence (1:50 - 1:200)
- Western blot (1:500 - 1:2000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
IRF2 antibody was raised against recombinant full length protein of human IRF2
Recognizes endogenous levels of IRF2 protein.
Western blot analysis of IRF2 expression in SW480 (A), NIH3T3 (B) whole cell lysates. Immunohistochemical analysis of IRF2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of IRF2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
For research use only.