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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IRF2 Antibody LS‑C667867
IRF2 antibody LS-C667867 is an unconjugated rabbit polyclonal antibody to human IRF2. Validated for ICC, IF, IHC and WB.
Catalog
Size
Price
LS-C667867-50
50 µl (1 mg/ml)
$235
LS-C667867-100
100 µl (1 mg/ml)
$275

Popular IRF2 Products

Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Staining (2?g/ml) of Jurkat lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Applications: Western blot
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep
Applications: Western blot
Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA

Product Description

IRF2 antibody LS-C667867 is an unconjugated rabbit polyclonal antibody to human IRF2. Validated for ICC, IF, IHC and WB.
About IRF2
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. P14316 NM_002199 NP_002190.2

IRF2 Antibody, IRF-2 Antibody, Interferon regulatory factor 2 Antibody


Specifications

Target
Human IRF2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity chromatography
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • IHC - Paraffin
  • ICC
  • Immunofluorescence (1:50 - 1:200)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IRF2 antibody was raised against recombinant full length protein of human IRF2
Specificity
Recognizes endogenous levels of IRF2 protein.
Usage
Western blot analysis of IRF2 expression in SW480 (A), NIH3T3 (B) whole cell lysates. Immunohistochemical analysis of IRF2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Immunofluorescent analysis of IRF2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Presentation
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.

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Images

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot

Immunohistochemistry - Paraffin

Immunofluorescence

Western blot


Requested From: United States
Date Requested: 9/22/2018

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