LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
IRF2 Antibody (clone 3B5) LS‑C133274
IRF2 antibody LS-C133274 is an unconjugated mouse monoclonal antibody to human IRF2. Validated for ELISA, IF, IHC and WB.
Catalog
Size
Price
LS-C133274-100
100 µg
$355

Popular IRF2 Products

Antibody
Species: Human, Mouse
Applications: Western blot, ELISA
Staining (2?g/ml) of Jurkat lysate (RIPA buffer, 30?g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Species: Human, Monkey, Mouse, Rat, Dog, Hamster, Horse
Applications: Western blot
Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Chimpanzee, Orangutan, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig, Rabbit, Sheep
Applications: Western blot
Immunohistochemistry - Paraffin Image
Species: Human
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot

Product Description

IRF2 antibody LS-C133274 is an unconjugated mouse monoclonal antibody to human IRF2. Validated for ELISA, IF, IHC and WB.

Specifications

Target
Human IRF2
Synonyms
IRF2, IRF-2, Interferon regulatory factor 2
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [3B5]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (3 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IRF2 antibody was raised against iRF2 (NP_002190, 216 a.a. ~ 316 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human IRF2
Usage
Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
About IRF2
Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. P14316 NM_002199 NP_002190.2


Publications (0)


Customer Reviews (0)


Images

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.
IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.

Western blot

Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.
Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.
IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.

Western blot

Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.
Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.
IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.

Western blot

Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.
Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.

Immunohistochemistry

Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]
Immunoperoxidase of monoclonal antibody to IRF2 on formalin-fixed paraffin-embedded human leiomyosarcoma. [antibody concentration 3 ug/ml]

Immunofluorescence

Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence of monoclonal antibody to IRF2 on HeLa cell. [antibody concentration 10 ug/ml]

Western blot

IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.
IRF2 monoclonal antibody (M02), clone 3B5 Western blot of IRF2 expression in HeLa NE.

Western blot

Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.
Western blot of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody (M02), clone 3B5.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged IRF2 is approximately 0.1 ng/ml as a capture antibody.

Requested From: United States
Date Requested: 11/14/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy