MAR antibody LS-C116008 is an unconjugated rabbit polyclonal antibody to MAR (IRF1) from mouse, rat and rabbit. Validated for WB.
Mouse, Rat, Rabbit
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
IRF1 / MAR antibody was raised against synthetic peptide located between aa251-300 of mouse Irf1 (P15314, NP_032416). Percent identity by BLAST analysis: Mouse, Rat (100%); Human, Gorilla, Gibbon, Baboon, Monkey (92%); Ferret, Sheep, Panda, Bovine (90%); Marmoset, Rabbit, Guinea pig, Armadillo (85%); Galago (84%).
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.