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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C152922-100 100 µl $475 
Integrin Alpha5 Beta1 Antibody - IHC: Rat anti-Mouse Integrin alpha5beta1 (INTEGRIN A5/B1, RT X MS-100UL) staining of mouse gut.

Monoclonal Rat anti‑Mouse Integrin Alpha5 Beta1 Antibody (IHC) LS‑C152922

Monoclonal Rat anti‑Mouse Integrin Alpha5 Beta1 Antibody (IHC) LS‑C152922

Antibody:
Integrin Alpha5 Beta1 Rat anti-Mouse Monoclonal Antibody
Application:
IHC, IHC-Fr, IP, Flo, Func
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$475
LS-C152922-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Integrin Alpha5 Beta1 Rat anti-Mouse Monoclonal Antibody
Application:
IHC, IHC-Fr, IP, Flo, Func
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Integrin Alpha5 Beta1 antibody LS-C152922 is an unconjugated rat monoclonal antibody to mouse Integrin Alpha5 Beta1. Validated for Flow, Func, IHC and IP.
Target
Mouse Integrin Alpha5 Beta1
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2c Monoclonal
Conjugations
Unconjugated
Purification
Ascites
Modifications
Unmodified
Immunogen
Mouse embryonal carcinoma P19 cells.
Specificity
This antibody was raised against mouse integrin VLA-5 (alpha5beta1), a fibronectin receptor. The antibody was prepared by fusion of spleen cells from a Fisher rat immunized with mouse embryonal carcinoma P19 cells with myeloma P3X63Ag9.653 cells. Specificity of the antibody was verified by its ability to immunoprecipitate alpha5beta1 heterodimers from (125)-I-surface labeled cells, reciprocal depletion of VLA-5 antigen from cell lysate with antiserum against the cytoplasmic domain of a5 integrin subunit, and immunoprecipitation of VLA-5 integrin from cells known to express this integrin. Cell lines tested to express alpha5beta1, include: P19 cells, mouse melanoma B16F1 cells, mouse squamous carcinoma SCC cells and thymocytes. Characterization by immunoprecipitation was typically analyzed by SDS-PAGE using 6% gels under non-reducing conditions
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Usage
Inhibition of cell adhesion to fibronectin: 10 ul/mL. Note that where cell adhesion to fibronectin involving other fibronectin receptors, such as VLA-4 (alpha4beta1) integrin, is involved, inhibition of cell adhesion by BMC5 alone may not be complete. Imm
Presentation
Ascites fluid.
Storage
Store at -20°C. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/27/2020