Target
Human Integrin Alpha2 Beta1
Reactivity
Human, Pig
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Conjugations
Unconjugated.
Also available conjugated with
AP,
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
Human fibrosarcoma HT1080 cells.
Specificity
Recognizes the human collagen receptor VLA-2 (alpha 2 beta 1) integrin complex. Species cross-reactivity: porcine.
Applications
- IHC
- IHC - Frozen (5 µg/ml)
- Immunoprecipitation
- Flow Cytometry (10 µg/ml)
- Functional Assay (10 µg/ml)
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Usage
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry, and Inhibition of VLA-2 mediated cell adhesion to collagen and laminin. Immunoprecipitation: 2-5 ug (lysate of 1E6 cell equivalent). Flow Cytometry: 10 ug/ml. Immunohistochemistry (Frozen tissue sections): 5 ug/ml. Inhibition: 10 ug/ml. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.1, No preservatives added
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee