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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C152920-100 100 µg $455 
Integrin Alpha1 Beta1 Antibody - (INTEGRIN A1/B1, MS X RT-100UG) Staining of integrin alpha1beta1 in rat lung tissue.
Integrin Alpha1 Beta1 Antibody - IHC: (INTEGRIN A1/B1, MS X RT-100UG) Staining of integrin alpha1beta1 in rat heart tissue.
Integrin Alpha1 Beta1 Antibody - (INTEGRIN A1/B1, MS X RT-100UG) Staining of integrin alpha1beta1 in rat lung tissue.
Integrin Alpha1 Beta1 Antibody - IHC: (INTEGRIN A1/B1, MS X RT-100UG) Staining of integrin alpha1beta1 in rat heart tissue.
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Monoclonal Integrin Alpha1 Beta1 Antibody (IHC) LS‑C152920

Monoclonal Integrin Alpha1 Beta1 Antibody (IHC) LS‑C152920

Antibody:
Mouse Monoclonal to Rat Integrin Alpha1 Beta1
Application:
IHC, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$455 
LS-C152920-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Rat Integrin Alpha1 Beta1
Application:
IHC, IHC-Fr, IP, Flo
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Integrin Alpha1 Beta1 antibody LS-C152920 is an unconjugated mouse monoclonal antibody to rat Integrin Alpha1 Beta1. Validated for Flow, IHC and IP.
Target
Rat Integrin Alpha1 Beta1
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • Western blot
Immunogen
Rat pheochromocytoma PC12 cells with myeloma P3X63ag9.653 cells.
Specificity
Antibody MAB2513 is directed against rat integrin a1b1 (VLA-1), a receptor for collagen and laminin. Antibody specificity has been confirmed by 1) its ability to immunoprecipitate beta1 (Mr ~200 kD) and b1 (Mr ~120 kD) from (125)I-surface labeled PC12 cells and 2) reciprocal depletion of VLA-1 antigen from cell lysate with antiserum against the cytoplasmic domain of a1 integrin subunit. Analysis of immunoprecipitation by SDS-PAGE was carried out under non-reducing conditions using a 6% gel. The antibody will not recognize the individual integrin subunits, only the VLA-1 complex. The antibody is not effective in western blots of VLA-1 for this reason, only immunoprecipitation or staining (acetone fixed only)
Usage
Immunoprecipitation of VLA-1 integrin from detergent cell lysates. Suggested dilution: 2 g per 10E6 cell equivalent. Flow cytometry: 10 ug/ml. Immunohistochemistry: Detects VLA-1 in acetone-fixed frozen tissue sections from gut, heart, and lungs. Suggesti
Presentation
0.02 M PB, pH 7.6, 0.25 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 2/25/2020