Human Integrin Alpha V Beta 3

Mouse

Human, Monkey, Bovine, Dog, Pig, Rabbit, Chicken, Avian (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

LM609

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Protein A purified

Unmodified

Purified adhesion receptor from the human melanoma cell line M21.

Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species cross-reactivity: bovine, chicken, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.

• IHC
• IHC - Frozen
• ICC
• Immunofluorescence (5 - 10 µg/ml)
• Immunoprecipitation
• Flow Cytometry

• IHC - Paraffin

Suitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Immunoprecipitation: Precipitates integrin alpha V beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD corresponding to the alpha V subunit and at ~105kD corresponding to the beta3 subunit. Flow Cytometry: 1-3 ug/0.5x1E6cells in 100 ul volume. Immunofluorescence: 5-10 ug/ml for staining of bFGF-treated 6mm cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4% paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Blocking: Inhibits adhesion of cells to vitronectin coated surfaces at 10-25 ug/ml. Positive control: Human myeloma cell lines M21 and MoalphaV.

PBS, pH 7.1, No preservatives added

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.