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| Catalog Number | Size | Price |
|---|---|---|
| LS-C60064-100 | 100 µg (1.52 mg/ml) | $567 |
![ING5 Antibody - Anti-p28 ING5 Antibody - Western Blot. Western blot analysis is shown using Affinity Purified anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kD corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Incubation was overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/ing5-antibody-aa125-150-ls-c60064/62765_49231.jpg)
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Polyclonal Goat anti‑Human ING5 Antibody (aa125‑150, WB) LS‑C60064
Polyclonal Goat anti‑Human ING5 Antibody (aa125‑150, WB) LS‑C60064
Note: This antibody replaces LS-C70700
Antibody:
ING5 Goat anti-Human Polyclonal (aa125-150) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ING5 Goat anti-Human Polyclonal (aa125-150) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Specifications
Description
ING5 antibody LS-C60064 is an unconjugated goat polyclonal antibody to human ING5 (aa125-150). Validated for ELISA and WB.
Host
Goat
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).
Epitope
aa125-150
Specificity
Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.
Applications
- Western blot (1:500 - 1:1000)
- ELISA (1:4000 - 1:16000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kD or 26 kD in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: -20°C; Long term: -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ING5
Publications (0)
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Featured Products
Source:
Wheat
Tag:
GST, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/15/2024
Date Requested: 4/15/2024
