Human IL4R / CD124

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

25463 (49/20)

Unconjugated

Protein A purified

Unmodified

Sf 21-derived, recombinant human interleukin 4 receptor, extracellular domain.

Extracellular Domain

Recognizes human Interleukin-4 receptor subunit alpha, also known as CD124. CD124 is a 825 amino acid ~90kD type I single pass transmembrane glycoprotein bearing a single domain. Interleukin-4 Receptor form a heterodimeric complex with the, referred to as the IL-4 type-1 complex. The alpha subunit can also form the type-II complex through association with the IL-13 receptor alpha 1 subunit. The IL-4R complex acts as a receptor for both interleukin-4 and interleukin-13. IL-4 R is expressed by B cells, T cells, monocytes and macrophages. Is reported to block human cell surface IL-4 receptor mediated bioactivities induced by IL-4 or IL-13. An ND50 was observed at approximately 5-20 ng/ml in the presence of 0.2 ng/ml of IL-4 using the factor dependant human erythroleukemic cell line, TF-1.

• IHC
• IHC - Paraffin (1:40 - 1:125)
• Western blot (1:250 - 1:1000)
• Flow Cytometry (1:20 - 1:80)
• ELISA (1:500 - 1:2000)

Western Blot: Will detect human IL-4 R under non-reducing conditions.

PBS, 0.5% trehalose

Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P24394 NCBI: NM_000418 NP_000409.1