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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone PC61.5.3, PE) LS‑C112608
CD25 antibody LS-C112608 is a PE-conjugated rat monoclonal antibody to mouse CD25 (IL2RA). Validated for Flow, Func, IHC and IP.
Catalog
Size
Price
LS-C112608-100
100 µg
$245
Description
CD25 antibody LS-C112608 is a PE-conjugated rat monoclonal antibody to mouse CD25 (IL2RA). Validated for Flow, Func, IHC and IP.
Target
Mouse IL2RA / CD25
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [PC61.5.3]
Conjugations
Phycoerythrin
Purification
Purified
Modifications
Unmodified
Applications
  • IHC - Frozen
  • Immunoprecipitation
  • Flow Cytometry
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL2RA / CD25 antibody was raised against b6.1 CTL cell line
Specificity
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4<sup>+</sup> T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Usage
The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 2 ug/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 15 mM Sodium Azide
Storage
Store in the dark at 2°C to 8°C. Do not freeze. Protect from light.
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Requested From: United States
Date Requested: 12/9/2018
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