CD25 antibody LS-C45156 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for ELISA, Flow and IHC. Cited in 1 publication.
(tested or 100% immunogen sequence identity)
Ion exchange chromatography
- IHC - Frozen
- Flow Cytometry (1:100)
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IL2RA / CD25 antibody was raised against rat interleukin-2 receptor.
Recognizes the rat CD25 cell surface antigen, a 55kD molecules that is the low affinity alpha chain of the IL-2 receptor. Recognizes a different epitope on CD25 to the OX39 clone (MCA273R). Clone NDS61 blocks IL-2 induced cell proliferation.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Store at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
For research use only.
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