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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone NDS 61, Azide‑free) LS‑C45156
CD25 antibody LS-C45156 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for ELISA, Flow, Func and IHC. Cited in 1 publication.
Catalog
Size
Price
LS-C45156-1000
1000 µg (1 mg/ml)
$875
Description
CD25 antibody LS-C45156 is an unconjugated mouse monoclonal antibody to rat CD25 (IL2RA). Validated for ELISA, Flow, Func and IHC. Cited in 1 publication.
Target
Rat IL2RA / CD25
Host
Mouse
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [NDS 61]
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Azide-free
Applications
  • IHC
  • IHC - Frozen
  • Flow Cytometry (1:100)
  • ELISA
  • Functional Assay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL2RA / CD25 antibody was raised against rat interleukin-2 receptor.
Specificity
Recognizes the rat CD25 cell surface antigen, a 55kD molecules that is the low affinity alpha chain of the IL-2 receptor. Recognizes a different epitope on CD25 to the OX39 clone (MCA273R). Clone NDS61 blocks IL-2 induced cell proliferation.
Usage
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Publications (1)

1
Inhibition of Pediatric Glioblastoma Tumor Growth by the Anti-Cancer Agent OKN-007 in Orthotopic Mouse Xenografts. Coutinho de Souza P, Mallory S, Smith N, Saunders D, Li XN, McNall-Knapp RY, Fung KM, Towner RA. PloS one. 2015 10:e0134276. (WB, Flo; Human, Rat) [Full Text Article] [PubMed:26248280] [PMC:PMC4527837]

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Requested From: United States
Date Requested: 12/9/2018
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