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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone ITYV) LS-C140316

Antibody

IL2RA / CD25 Antibody (clone ITYV) LS-C140316

Antibody:
Mouse Monoclonal to Human IL2RA / CD25
Application:
Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$275 
LS-C140316-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human IL2RA / CD25
Application:
Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
CD25 antibody LS-C140316 is an unconjugated mouse monoclonal antibody to human CD25 (IL2RA). Validated for Flow.

Target
Human IL2RA / CD25
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
ITYV
Conjugations
Unconjugated. Also available conjugated with FITC, Biotin, PE.
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Immunogen
IL2RA / CD25 antibody was raised against pHA-activated human T-lymphocytes.
Specificity
Human IL2RA / CD25
Usage
PBMC: Add 10 ul of antibody/10^6 PBMC in 100 ul PBS. Mix gently and incubate for 15 minutes at 2 to 8°C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ul of antibody/100 ul of whole blood. Mix gently and incubate for 15 minutes at room temperature 20°C. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Presentation
PBS, 0.08% Sodium Azide
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

Publications (0)

Reviews (0)

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Requested From: United States
Date Requested: 6/20/2019
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