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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone 3C7, Biotin) LS-C196224

Antibody

IL2RA / CD25 Antibody (clone 3C7, Biotin) LS-C196224

Antibody:
Rat Monoclonal to Mouse IL2RA / CD25
Application:
IP, Flo, Inhb
Reactivity:
Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$455 
LS-C196224-500
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Mouse IL2RA / CD25
Application:
IP, Flo, Inhb
Reactivity:
Mouse
Format:
Biotin, Unmodified

Specifications

Description
CD25 antibody LS-C196224 is a biotin-conjugated rat monoclonal antibody to mouse CD25 (IL2RA). Validated for Flow, Inhb and IP.

Target
Mouse IL2RA / CD25
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
3C7
Conjugations
Biotin
Modifications
Unmodified
Applications
  • Immunoprecipitation
  • Flow Cytometry
  • Inhibition
Immunogen
IL2RA / CD25 antibody was raised against alpha chain IL-2 receptor.
Specificity
Mouse IL2RA / CD25
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, Sodium Azide
Storage
Store at 4°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Immunohistochemical analysis of EGR1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
Reactivity: Human
Range: 40-10000 pg/ml
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRAS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRAS.
Species: Human
Applications: Western blot
Reactivity: Human
Range: 0.312-20 ng/ml

Requested From: United States
Date Requested: 6/16/2019
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