CD25 antibody LS-C390441 is an unconjugated mouse monoclonal antibody to human CD25 (IL2RA). Validated for Flow and IF.
(tested or 100% immunogen sequence identity)
Also available Unmodified
IL2RA / CD25 antibody was raised against stimulated human leukocytes were used as the immunogen for the CD25 antibody.
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 55kDa, identified as CD25 (Workshop IV. Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R b-chain, p75) and CD132 (common g-chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This mAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25. Optimal dilution of the CD25 antibody should be determined by the researcher.