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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL2RA / CD25 Antibody (clone 143‑13, Azide‑free) LS‑C390441
CD25 antibody LS-C390441 is an unconjugated mouse monoclonal antibody to human CD25 (IL2RA). Validated for Flow and IF.
Catalog
Size
Price
LS-C390441-100
100 µg (1 mg/ml)
$415
Description
CD25 antibody LS-C390441 is an unconjugated mouse monoclonal antibody to human CD25 (IL2RA). Validated for Flow and IF.
Target
Human IL2RA / CD25
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal [143-13]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Azide-free. Also available Unmodified.
Applications
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
  • (applications tested for the base form of this product only)
Immunogen
IL2RA / CD25 antibody was raised against stimulated human leukocytes were used as the immunogen for the CD25 antibody.
Specificity
Human IL2RA / CD25
Usage
The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Recognizes a protein of 55kDa, identified as CD25 (Workshop IV. Code A27). CD25 is expressed on activated T- and B-cells and activated monocytes/macrophages. With respect to lymphomas, CD25 is present on malignant cells of Hodgkins disease, HTLV-1+ adult T-cell leukemia, cutaneous T-cell lymphoma, and hair cell leukemia. Increased levels of soluble CD25 are observed in the leukemias/lymphomas and inflammatory/ autoimmune diseases. CD25 molecule alone appears to function as a low affinity receptor and associates with CD122 (IL-2R b-chain, p75) and CD132 (common g-chain) to form the high affinity IL-2 receptor complex. CD25 antibodies detect three epitope regions, A, B and C. This mAb recognizes the epitope B, which is located at residue 3-104 of CD25 and can effectively block IL-2 binding to CD25. Optimal dilution of the CD25 antibody should be determined by the researcher.
Presentation
Sterile Solution PBS
Storage
Store at 2°C to 8°C.
Restrictions
For research use only.
About IL2RA / CD25
P01589 NM_000417 NP_000408.1

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Requested From: United States
Date Requested: 12/9/2018
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