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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL1RN Antibody IHC‑plus™ LS‑B7435
Note: This antibody replaces LS-C104493, LS-C188017
IL1RN antibody LS-B7435 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, IHC, Neut and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7435-50
50 µg
Unavailable

Popular IL1RN Products

Antibody
Species: Human
Applications: ELISA
Antibody
Species: Human
Applications: ELISA
Human peripheral blood mononuclear cells were stimulated with intracellularly stained with FITC mouse IgG1 isotype control. The cells were surface stained with PE anti-human CD14 and intracellularly stained with FITC anti-human IL-1RA (CRM17). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
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Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

IL1RN antibody LS-B7435 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, IHC, Neut and WB. Tested on 20 paraffin-embedded human tissues.
About IL1RN
IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. P18510 NM_000577 NP_000568.1

IL1RN Antibody, Anakinra Antibody, DIRA Antibody, IL-1ra Antibody, IL-1RN Antibody, IL1F3 Antibody, IL1RA Antibody, MVCD4 Antibody, IL1 inhibitor Antibody, IL1RN (IL1F3) Antibody, IRAP Antibody, ICIL-1RA Antibody, IL-1ra3 Antibody


Specifications

Target
Human IL1RN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (2.5 - 3.7 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL1RN antibody was raised against e.coli derived recombinant Human IL-1RA.
Specificity
Human IL-1RA
Usage
Immunohistochemistry: LS-B7435 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human IL-1RA (50 ng/ml) and Human IL-1alpha (50 pg/ml), a concentration of 2.5-3.7 ug/ml of this antibody is required. ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-1RA (LS-C104474) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL-1RA is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
50 µl Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435

Western blot

Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435
Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435

Immunohistochemistry

Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435

Western blot

Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435
Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435

Immunohistochemistry

Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435

Western blot

Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435
Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435

Immunohistochemistry

Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.
Anti-IL1RN / IL-1RA antibody IHC of human skin, epidermis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7435 concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (non-reducing) of IL1RN / IL-1RA antibody LS-B7435

Western blot

Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435
Western Blot (reducing) of IL1RN / IL-1RA antibody LS-B7435

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435
Sandwich ELISA of IL1RN / IL-1RA antibody LS-B7435

Requested From: 
Date Requested: 7/18/2018

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