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Catalog Number Size Price
LS-C18829-1 1 mg (1 mg/ml) $964 
Antibody

Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑C18829

Polyclonal Rabbit anti‑Human IL1RN Antibody (IHC, WB) LS‑C18829

Note: This antibody replaces LS-C70858
Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$964
LS-C18829-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL1RN Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL1RN antibody LS-C18829 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, Flow, IHC, Neut, RIA and WB.
Target
Human IL1RN
Synonyms
IL1RN | DIRA | IL-1ra | IL-1RN | IL1F3 | IL1RA | MVCD4 | IL1 inhibitor | IL1RN (IL1F3) | IRAP | ICIL-1RA | IL-1ra3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
DEAE chromatography
Modifications
Unmodified
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human sIL-1Ra produced in E.coli.
Specificity
This antibody also detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. The antibody does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. This antibody is primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra) but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra). IcIL-1Ra is found in many cells as constitutively expressed protein, particularly epithelial cells and keratinocytes. This antibody does not distinguish between sIL-1Ra and icIL-1Ra in ELISA formats or by immunohistochemistry. When used in immunoblot analysis, it will recognize 17,000 MW IL-1Ra in supernatants released from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS. However, in lysates of PBMC, icIL-1Ra is also found after 48-72 hours of culture. In monocytic cells, the initial IL-1Ra transcript is for sIL-1Ra which can be found almost exclusively intracellularly.
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:1000 - 1:5000)
  • Neutralization
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1RN
P18510 NM_000577 NP_000568.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/16/2022