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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL1RN Antibody LS‑C18829
Note: This antibody replaces LS-C70858
IL1RN antibody LS-C18829 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, Flow, IHC, RIA and WB.
Antibody
Catalog
Size
Price
LS-C18829-1000
1000 µg
$1,140

Popular IL1RN Products

Western blot of IL1RN expression in transfected 293T cell line by IL1RN monoclonal antibody (M03), clone 1H5.
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: ELISA
Human peripheral blood mononuclear cells were stimulated with intracellularly stained with FITC mouse IgG1 isotype control. The cells were surface stained with PE anti-human CD14 and intracellularly stained with FITC anti-human IL-1RA (CRM17). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: Flow Cytometry
Antibody
Species: Human
Applications: ELISA
IL1RN Antibody (RB18901) IHC of formalin-fixed and paraffin-embedded human Colon carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

IL1RN antibody LS-C18829 is an unconjugated rabbit polyclonal antibody to human IL1RN. Validated for ELISA, Flow, IHC, RIA and WB.
About IL1RN
IL1RN is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. P18510 NM_000577 NP_000568.1

IL1RN Antibody, Anakinra Antibody, DIRA Antibody, IL-1ra Antibody, IL-1RN Antibody, IL1F3 Antibody, IL1RA Antibody, MVCD4 Antibody, IL1 inhibitor Antibody, IL1RN (IL1F3) Antibody, IRAP Antibody, ICIL-1RA Antibody, IL-1ra3 Antibody


Specifications

Target
Human IL1RN
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified IgG
Modifications
Unmodified
Applications
  • IHC
  • Western blot
  • Flow Cytometry
  • ELISA (1:500 - 1:1000)
  • Radioimmunoassay
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL1RN antibody was raised against recombinant human IL1RN / IL-1RA.
Specificity
Recombinant human sIL-1Ra produced in E. coli.
Usage
This IgG fraction antibody of anti-Human IL-1 Receptor Antagonist has been tested for use in neutralizations, ELISA, radioimmunoassays, western blotting, immunohistochemistry and flow cytometry. Reactivity in other immunoassays is unknown. This product has been assayed by immunoblot using HRP Goat-anti-Rabbit IgG [H&L] (catalog no. LS-C60884) and TMB as a substrate. A working dilution range of 1:400 to 1:800 is suggested for this application. This product has been assayed by ELISA against IL-1 Receptor Antagonist using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:500 to 1:1000 is suggested for this product. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This product has been assayed by immunohistochemistry. A dilution of 1:400 or greater is suggested for this immunoassay. Either paraffin fixation or cryofixation can be used for immunohistochemistry. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.

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Requested From: United States
Date Requested: 9/25/2018

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