This antiserum against anti-Human IL-1 Receptor Type I (IL-1 RI) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by ELISA against soluble (extracellular) IL-1 RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884
) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1000 to 1:2000 is suggested for this product. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.