Human IL1R1

Rabbit

Human, Primate (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

IL-1 Receptor Type I Antibody was prepared by repeated immunizations with recombinant human IL-1R type I.

The antiserum is directed against the extracellular domain of the human IL-1R type I, but precipitates the intact receptor expressed on human cells. In general, this antibody also detects primate IL-1RI. The antibody does not recognize human IL-1, IL-1Ra or IL-1R type II. In ELISA formats and other immunoreactive assays, this antibody will detect the extracellular domain of the IL-1R type I (also called soluble IL-1R type I or ''decoy'' receptor) found in human body fluids, particularly in the circulation, urine and supernatants of cells and synovial fluid.

• Immunoprecipitation
• ELISA (1:1000 - 1:2000)
• Radioimmunoassay

This antiserum against anti-Human IL-1 Receptor Type I (IL-1 RI) has been tested for use in ELISA, radioimmunoassays and immunoprecipitation. Reactivity in other immunoassays is unknown. This product has been assayed for the ability to immunoprecipitate antigen. A dilution range of 1:400 to 1:800 is suggested for this immunoassay. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background. This product has been assayed by ELISA against soluble (extracellular) IL-1 RI using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (catalog no. LS-C60884) and ABTS as a substrate for 30 minutes at room temperature. A working dilution range of 1:1000 to 1:2000 is suggested for this product. This product has been assayed by radioimmunoassay against antigen. A dilution of 1:8000 is suggested for this immunoassay.

0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P14778 NCBI: NM_000877 NP_000868.1