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Catalog Number Size Price
LS-C18826-1 1 mg (1 mg/ml) $964 
IL1A / IL-1 Alpha Antibody - Western Blot of Rabbit Anti-Human IL-1 alpha Antibody. Lane 1: Opal Prestained Molecular Weight Ladder  Lane 2: IL-1 alpha 50ng. Lane 3: MEF WC lysate 10ng. Lane 4: MEF LPS stimulated 10ng.BlockOut Buffer for 30min at RT. Primary Antibody: Anti-IL-1a 1µg/mL overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP at 1:70, 000) for 30min at RT.

Polyclonal Rabbit anti‑Human IL1A / IL‑1 Alpha Antibody (IHC, WB) LS‑C18826

Polyclonal Rabbit anti‑Human IL1A / IL‑1 Alpha Antibody (IHC, WB) LS‑C18826

Antibody:
IL1A / IL-1 Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$964
LS-C18826-1
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL1A / IL-1 Alpha Rabbit anti-Human Polyclonal Antibody
Application:
IHC, WB, Flo, ELISA, Neut
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
IL-1 Alpha antibody LS-C18826 is an unconjugated rabbit polyclonal antibody to human IL-1 Alpha (IL1A). Validated for ELISA, Flow, IHC, Neut and WB.
Target
Human IL1A / IL-1 Alpha
Synonyms
IL1A | Hematopoietin-1 | IL-1 alpha | IL1 | IL1-ALPHA | IL1 Alpha | Interleukin-1 alpha | IL-1A | IL1F1 | Pro-interleukin-1-alpha | Preinterleukin 1 alpha | Interleukin 1, alpha
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Immunogen
The whole rabbit serum used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human IL-1a produced in E.coli.  The MW of the recombinant IL-1a was 17,000.  This is the cleavage site generated by the IL-1b converting enzyme (ICE, capase-1).
Applications
  • IHC
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:1000 - 1:5000)
  • Neutralization
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This IgG fraction antibody of anti-Human IL-1a has been tested for use in neutralizations, ELISA, immunohistochemistry, flow cytometry and immunoblotting. It recognizes the 17000 MW mature IL-1a. Reactivity in other immunoassays is unknown.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL1A / IL-1 Alpha
P01583 NM_000575 NP_000566.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/13/2022