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Catalog Number	Size	Price
LS-C313463-10	10 µg	$318
LS-C313463-100	100 µg (0.5 mg/ml)	$470

IL18 Antibody - IHC analysis of IL-18 using anti-IL-18 antibody. IL-18 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL-18 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IL18 Antibody - WB of IL18 antibody. Lane 1: Recombinant Mouse IL18 Protein 10ng. Lane 2: Recombinant Mouse IL18 Protein 5ng. Lane 3: Recombinant Mouse IL18 Protein 2.5ng. Lane 4: Recombinant Mouse IL18 Protein 1.25ng..

IL18 Antibody - Western blot analysis of IL-18 using anti-IL-18 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Recombinant Mouse IL18 Protein 10ng, Lane 2: Recombinant Mouse IL18 Protein 5ng, Lane 3: Recombinant Mouse IL18 Protein 2. 5ng, Lane 4: Recombinant Mouse IL18 Protein 1. 25ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-18 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL-18 at approximately 21KD. The expected band size for IL-18 is at 21KD.

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Polyclonal Rabbit anti‑Mouse IL18 Antibody (aa36‑192, WB) LS‑C313463

Name: Anti-IL18 Antibody | Rabbit anti-Mouse Polyclonal ELISA,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-C313463-10
Price: 318.0000 USD
Availability: InStock

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Note: This antibody replaces LS-C388693, LS-C388679

Antibody:

IL18 Rabbit anti-Mouse Polyclonal (aa36-192) Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

$318

LS-C313463-10

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

IL18 Rabbit anti-Mouse Polyclonal (aa36-192) Antibody

Application:

WB, ELISA

Reactivity:

Mouse

Format:

Unconjugated, Unmodified

Specifications

Description

IL18 antibody LS-C313463 is an unconjugated rabbit polyclonal antibody to mouse IL18 (aa36-192). Validated for ELISA and WB.

Target

Mouse IL18

Synonyms

See All IL18 Antibodies

Host

Rabbit

Reactivity

Mouse (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunogen affinity purified

Modifications

Unmodified

Immunogen

E. coli-derived mouse IL-18 recombinant protein(Position: N36-S192).

Epitope

aa36-192

Applications

Western blot
ELISA

Presentation

Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500µg/ml.

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About IL18

Uniprot: Q14116 NCBI: NM_001562 NP_001553.1