Human IL17F

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2b Monoclonal

197301

Unconjugated

Protein G purified

Unmodified

E. coli-derived rhIL-17F corresponding to aa 31-163 according to Q96PD4.

Recognizes human IL-17F. In direct ELISAs and Western Blots this antibody does not cross-react with rhIL-17A, B, C, D, or E.

• Flow Cytometry
• ELISA

Suitable for use in ELISA and Flow Cytometry. The detection limit is ~25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry (Intracellular): 25 ug/ml. Add 10 ul to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. For intracellular staining to detect IL-17F, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. This antibody was tested using PMA and Ca2+ ionomycin-treated PBMCs. ELISA (Direct): 0.5-1.0 ug/ml. The detection limit is ~1 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

Reconstitute in 200 ul sterile PBS

Store at -20°C. After reconstitution, aliquot and store at -20°C. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q96PD4 NCBI: Q96PD4