IL17D | IL-27 | Interleukin 27 | Interleukin-17D | Interleukin-27 | IL-17D | IL-22 | Interleukin 17D
(tested or 100% immunogen sequence identity)
Protein G purified
Recombinant protein corresponding to aa25-205 from mouse Interleukin 17D expressed in E. coli (NP_665836).
Recognizes mouse IL-17D. In direct ELISAs and Western Blots this antibody shows no cross-reactivity with rhIL-17D, rmIL-17, rmIL-17B, rmIL-17C, rmIL-17E or rmIL-17F.
- Western blot
- Flow Cytometry
Western Blot, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect mouse IL-17D. Using a colorimetric detection system, the detection limit for rmIL-17D is approximately 5 ng/lane under non-reducing and reducing conditions. Use of this antibody under non reducing/reducing conditions is not recommended. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was validated for flow cytometry using CD4+ splenic lymphocytes. For intracellular staining to detect IL-17D, cells must first be fixed and permeabilized with 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding a secondary developing reagent such as goat anti-rat IgG conjugated to a fluorochrome. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse IL-17D. The detection limit for rmIL-17D is approximately 1 ng/well.
Lyophilized from PBS, 5% Trehalose
Reconstitute in 200 ul sterile PBS
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.