IL17D | IL-27 | Interleukin 27 | Interleukin-17D | Interleukin-27 | IL-17D | IL-22 | Interleukin 17D
(tested or 100% immunogen sequence identity)
Protein G purified
E. coli-derived recombinant human Interleukin 27 (aa 18-202).
Recognizes human IL-27. Does not cross-react with rhIL-17, rhIL17B, rhIL-17C, rhIL-17E, or rhIL-17F. Species sequence homology: Mouse: 86%.
- IHC - Paraffin
- Western blot (1 - 2 µg/ml)
- Flow Cytometry (25 µg/ml)
- ELISA (0.5 - 1 µg/ml)
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Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and ELISA. Western Blot: 1-2 ug/ml. Detection limit is approximately 25 ng/lane under non-reducing conditions. Reducing conditions are not recommended. Flow Cytometry: 25 ug/ml. For intracellular staining, cells must be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Add 10 ul of the diluted solution to 1-5x10^5 cells in a total reaction volume not exceeding 200ul. Incubate for 30 minutes and then wash cells with 0.1% saponin prior to addition of a secondary developing reagent. Cells should be washed a final time in 0.1% saponin prior to flow cytometric analysis. Direct ELISA: 0.5-1 ug/ml. Detection limit is ~5 ng/well. Immunohistochemistry: 8-25 ug/ml with appropriate secondary reagents to detect IL-17D in paraffin-embedded normal heart tissue sections.
Lyophilized from PBS, 5% Trehalose
Reconstitute with sterile 40-50% glycerol, PBS.
Store at -20°C lyophilized. Once reconstituted, aliquot and store at -20°C.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.