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Antibodies > IL17A > LS-C662635
 
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Catalog Number Size Price
LS-C662635-10 10 µg $318 
LS-C662635-100 100 µg $470 
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Western blot analysis of IL17 using anti-IL17 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
IL17A Antibody - Western blot - Anti-IL17 Picoband Antibody
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Immunohistochemistry - Anti-IL17 Picoband Antibody
IL17A Antibody - Western blot analysis of IL17 using anti-IL17 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL17 at approximately 27KD. The expected band size for IL17 is at 17KD.
IL17A Antibody - Western blot - Anti-IL17 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse IL17A Antibody (IHC, WB) LS‑C662635

Polyclonal Rabbit anti‑Mouse IL17A Antibody (IHC, WB) LS‑C662635

Submit Review LSBio Guarantee
Antibody:
IL17A Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662635-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL17A Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Human, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IL17A antibody LS-C662635 is an unconjugated rabbit polyclonal antibody to IL17A from mouse. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived mouse IL17 recombinant protein (Position: A26-A158).
Specificity
Restricted to a subset of activated T-cells.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

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Featured Products

IL17A Antibody - Anti-IL-17 antibody IHC of human t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ IL17A Antibody LS‑B7397
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IL17A Antibody - Untreated (A) and PMA/ionomycin -stimulated (B) human PBMCs were stained using PE-conjugated mouse anti-human CD3 antibody and APC-conjugated antibody Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8) (APC) This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (APC) LS‑C37030
Species: Human
Applications: Flow Cytometry
IL17A Antibody - Positive Staining in Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
PathPlus™ IL17A Antibody (aa1‑75, clone 4K5F6) LS‑B8323
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IL17A Antibody - Western Blot (reducing) of IL-17 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody LS‑C104427
Species: Human
Applications: Western blot, ELISA
Product
IL17A Antibody (clone 41802, PE) LS‑C37031
Species: Human
Applications: Flow Cytometry
IL17A Antibody - Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (Biotin) LS‑C154151
Species: Rat, Mouse
Applications: IHC, Western blot, ELISA
See all IL17A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/24/2024
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