Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > IL17A > LS-C745269
 
Print Page  
Catalog Number Size Price
LS-C745269-25 25 µl (2.94 mg/ml) $304 
IL17A Antibody - Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
IL17A Antibody - Monoclonal anti-IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti-mouse IL-17A monoclonal antibody or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody.
IL17A Antibody - Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
IL17A Antibody - Monoclonal anti-IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti-mouse IL-17A monoclonal antibody or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody.
1 of 2
2 of 2

Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C745269

Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C745269

Submit Review LSBio Guarantee
Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C745269-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL17A antibody LS-C745269 is an unconjugated rat monoclonal antibody to mouse IL17A. Validated for ELISA, Flow and WB.
Target
Mouse IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
20B4.G10.F5
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:500)
  • ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

Publications (0)

Submit a Publication for LS-C745269

Customer Reviews (0)

Submit a review for LS-C745269

Featured Products

IL17A Antibody - Anti-IL-17 antibody IHC of human t lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ IL17A Antibody LS‑B7397
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
IL17A Antibody - Untreated (A) and PMA/ionomycin -stimulated (B) human PBMCs were stained using PE-conjugated mouse anti-human CD3 antibody and APC-conjugated antibody Interleukin 17 (IL-17, CTLA8, IL-17A, Interleukin-17A precursor, Cytotoxic T-lymphocyte-associated antigen 8) (APC) This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (APC) LS‑C37030
Species: Human
Applications: Flow Cytometry
IL17A Antibody - Positive Staining in Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
PathPlus™ IL17A Antibody (aa1‑75, clone 4K5F6) LS‑B8323
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
IL17A Antibody - Western Blot (reducing) of IL-17 antibody. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody LS‑C104427
Species: Human
Applications: Western blot, ELISA
Product
IL17A Antibody (clone 41802, PE) LS‑C37031
Species: Human
Applications: Flow Cytometry
IL17A Antibody - Biotin Rabbit Anti-Rat IL-17A Antibody - Western Blot. Western Blot showing detection of Rat IL-17A. 50 ng of Rat IL-17A (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 5% Blotto (B501-0500) 30 min at 20?, Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated Anti-Rat IL-17A (RABBIT) Antibody Biotin Conjugated - 212-406-B32 secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070). HRP Streptavidin (p/n S000-03) was used at 1:40000 in MB-070 for 30 min at 20? and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 15 kD molecular weight position expected for Rat IL-17A. This image was taken for the unconjugated form of this product. Other forms have not been tested.
IL17A Antibody (Biotin) LS‑C154151
Species: Rat, Mouse
Applications: IHC, Western blot, ELISA
See all IL17A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com