Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C745269-25 25 µl (2.94 mg/ml) $275 
IL17A Antibody - Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
IL17A Antibody - Monoclonal anti-IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti-mouse IL-17A monoclonal antibody or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody.
IL17A Antibody - Western Blot showing detection of Mouse IL-17A. 100 ng of Mouse IL-17A was run on a 4-20% gel and transferred to 0.45 µm nitrocellulose. After blocking with 1% BSA-TTBS 30 min at 20°C, Anti-Mouse IL-17A (RAT) Antibody was used at 1:1000 in 1% BSA-TTBS over night at 4°C. Peroxidase conjugated Rabbit Anti-mouse secondary antibody was diluted in Blocking Buffer for Fluorescent Western Blotting at 1:40,000 for 30 min at 20°C and imaged using the Bio-Rad VersaDoc 4000 MP. Band indicates correct 23 kDa molecular weight position expected for Mouse IL-17A.
IL17A Antibody - Monoclonal anti-IL-17A was used to detect IL17A and separate Mouse CD4+ Cells by flow cytometry. Mouse CD4+ T cells were isolated from freshly dissected spleen by centrifugation in T cell separation media and selected by magnetic separation. Cells were grown on plates coated with anti-CD3 antibody, and stimulated with: 5 µg/mL anti-CD28, 10 ng/mL IL-1beta, 50 ng/mL mouse IL-6, 1 ng/mL TGFbeta1 and 10 µg/mL anti-mouse IFN gamma over 8-10 days of culture. Cells were incubated for 15-20 minutes with addition of rat anti-mouse CD4 APC at a concentration of 0.125 µg/mL, washed, fixed and permeabilized and incubated with Rat anti-mouse IL-17A monoclonal antibody or controls as shown. Cells were washed, incubated in streptavidin conjugated PE, fixed and analyzed by Flow cytometry. Shown here are results for monoclonal anti mouse IL-17A antibody.
1 of 2
2 of 2

Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C745269

Monoclonal Rat anti‑Mouse IL17A Antibody (clone 20B4.G10.F5, WB) LS‑C745269

Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745269-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
IL17A Rat anti-Mouse Monoclonal (20B4.G10.F5) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
IL17A antibody LS-C745269 is an unconjugated rat monoclonal antibody to mouse IL17A. Validated for ELISA, Flow and WB.
Target
Mouse IL17A
Synonyms
IL17A | CTLA-8 | CTLA8 | Interleukin-17A | Interleukin 17A | IL-17 | IL-17A | IL17
Host
Rat
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
20B4.G10.F5
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Unmodified
Immunogen
This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.
Specificity
This antibody is specific for mouse IL-17A protein. Cross-reactivity with IL-17A from other sources has not been determined.
Applications
  • Western blot (1:1000)
  • Flow Cytometry (1:500)
  • ELISA (1:10000)
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL17A
Q16552 NM_002190 NP_002181.1

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/10/2021