Pig, Bovine, Sheep
(tested or 100% immunogen sequence identity)
Protein A purified
- Western blot (1.5 - 2 µg/ml)
IL13 antibody was raised against full length recombinant protein produced in yeast, corresponding to mature swine IL-13 protein.
This antibody is specific for swine IL-13 protein. A BLAST analysis was used to suggest reactivity with IL-13 from swine sources based on 100% homology with the immunizing sequence. The following homologies may indicate chances of cross-reactivity when amino acid similarity and not just exact sequence is accounted: 89-90% homology to IL-13 from sheep, dolphin, beluga, and bovine, 83-88% to human and certain monkey IL-13, 80-82% to dusky titi, macaque, baboon, mangabey, dog, llama, camel, and galago, 75-78% to horse, and 67-72% to rat, mouse, and gerbil. Cross-reactivity with IL-13 from other sources has not been determined.
IL-13 antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 13.2 kD in size corresponding to swine IL-13 protein by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
100 µl deionized water
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.