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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL13 Antibody IHC‑plus™ LS‑B7417
Note: This antibody replaces LS-C104383
IL13 antibody LS-B7417 is an unconjugated rabbit polyclonal antibody to human IL13. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7417-50
50 µg
$425
Description
IL13 antibody LS-B7417 is an unconjugated rabbit polyclonal antibody to human IL13. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human IL13
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.1 - 0.2 µg/ml)
  • ELISA (0.5 - 2 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
IL13 antibody was raised against e.coli derived recombinant Human IL-13.
Specificity
Human IL-13
Usage
Immunohistochemistry: LS-B7417 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-13 (2 ng/ml), a concentration of 0.12-0.15 ug/ml of this antibody is required. ELISA: To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (LS-C104340) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.
Presentation
Lyophilized from PBS, pH 7.2
Reconstitution
50 µl Sterile water
Storage
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to 2 weeks at 2°C to 8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IL13
P35225 NM_002188 NP_002179.2

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Images

Immunohistochemistry

Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL-13 antibody
Western Blot (non-reducing) of IL-13 antibody

Western blot

Western Blot (reducing) of IL-13 antibody
Western Blot (reducing) of IL-13 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-13 antibody
Sandwich ELISA of IL-13 antibody

Immunohistochemistry

Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL-13 antibody
Western Blot (non-reducing) of IL-13 antibody

Western blot

Western Blot (reducing) of IL-13 antibody
Western Blot (reducing) of IL-13 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-13 antibody
Sandwich ELISA of IL-13 antibody

Immunohistochemistry

Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL-13 antibody
Western Blot (non-reducing) of IL-13 antibody

Western blot

Western Blot (reducing) of IL-13 antibody
Western Blot (reducing) of IL-13 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-13 antibody
Sandwich ELISA of IL-13 antibody

Immunohistochemistry

Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Western blot

Western Blot (non-reducing) of IL-13 antibody
Western Blot (non-reducing) of IL-13 antibody

Western blot

Western Blot (reducing) of IL-13 antibody
Western Blot (reducing) of IL-13 antibody

Enzyme-Linked Immunosorbent Assay

Sandwich ELISA of IL-13 antibody
Sandwich ELISA of IL-13 antibody

Requested From: United States
Date Requested: 11/17/2018
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