The biotinylated 1316H antibody has been tested as the detection antibody in a sandwich ELISA (and ELISPOT assay) for analysis of mouse interleukin-13 (IL-13) in combination with the purified 13A (LS-C107457
) antibody for capture and recombinant mouse IL-13 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25-1.0 ug/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 1000 pg/ml - 8 pg/ml should be included in each ELISA plate. For intracellular staining of mouse IL-13, the fluorochrome labeled 13A antibody is recommended. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.