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Catalog Number Size Price
LS-C662069-10 10 µg $318 
LS-C662069-100 100 µg $470 
IL12B / IL12 p40 Antibody - Immunohistochemistry - Anti-IL12B/Il 12 Picoband Antibody
IL12B / IL12 p40 Antibody - Western blot analysis of IL12B using anti-IL12B antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
IL12B / IL12 p40 Antibody - Western blot - Anti-IL12B/Il 12 Picoband Antibody
IL12B / IL12 p40 Antibody - Immunohistochemistry - Anti-IL12B/Il 12 Picoband Antibody
IL12B / IL12 p40 Antibody - Western blot analysis of IL12B using anti-IL12B antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL12B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4?, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IL12B at approximately 37-45KD. The expected band size for IL12B is at 37KD.
IL12B / IL12 p40 Antibody - Western blot - Anti-IL12B/Il 12 Picoband Antibody
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Polyclonal Rabbit anti‑Rat IL12B / IL12 p40 Antibody (IHC, WB) LS‑C662069

Polyclonal Rabbit anti‑Rat IL12B / IL12 p40 Antibody (IHC, WB) LS‑C662069

Antibody:
IL12B / IL12 p40 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662069-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL12B / IL12 p40 Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
IL12 p40 antibody LS-C662069 is an unconjugated rabbit polyclonal antibody to rat IL12 p40 (IL12B). Validated for ELISA, IHC and WB.
Target
Rat IL12B / IL12 p40
Synonyms
IL12B | CLMF p40 | IL-12 p40 | IL-12 subunit p40 | Interleukin-12 beta chain | Interleukin-12 subunit beta | IL12, subunit p40 | Interleukinl-12 | Interleukin 12, p40 | NKSF | CLMF | CLMF2 | IL-12B | NKSF2
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived rat IL12B recombinant protein (Position: M23-E250).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL12B / IL12 p40
P29460 NM_002187 NP_002178.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/23/2024