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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL10RA Antibody (C‑Terminus) LS‑C359025
IL10RA antibody LS-C359025 is an unconjugated rabbit polyclonal antibody to IL10RA from human and mouse. Validated for ICC, IF and WB.
Catalog
Size
Price
LS-C359025-30
30 µl (1 mg/ml)
$205
LS-C359025-100
100 µl (1 mg/ml)
$245
LS-C359025-200
200 µl (1 mg/ml)
$305

Popular IL10RA Products

Western blot of recombinant IL10RA.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human
Applications: IHC, Western blot
Antibody
Species: Mouse
Applications: Flow Cytometry, Functional Assay
Antibody
Species: Mouse
Applications: Flow Cytometry, Functional Assay
Antibody
Species: Human
Applications: Western blot
Antibody
Species: Mouse
Applications: Flow Cytometry

Product Description

IL10RA antibody LS-C359025 is an unconjugated rabbit polyclonal antibody to IL10RA from human and mouse. Validated for ICC, IF and WB.

Specifications

Target
Human IL10RA
Synonyms
IL10RA, CD210, CD210a, CD210 antigen, IBD28, IL-10R subunit 1, Il-10r, IL-10R subunit alpha, Interleukin 10 receptor, alpha, IL10R, Interleukin-10 receptor, HIL-10R, CDW210A, IL-10 receptor subunit alpha, IL-10R1, IL-10RA, Interleukin-10 receptors
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC (1:100 - 1:500)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:1000)
Immunogen
IL10RA antibody was raised against kLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human CD210a.
Blocking Peptide
LS-E41639 - Lyophilized - 1 mg - $145.00
Immunizing peptide used to generate LS-C359025. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Recognizes endogenous levels of CD210a protein.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only.
About IL10RA
IL10RA is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Q13651 NM_001558 NP_001549.2


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Images

Immunofluorescence

Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.
Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.
Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.
Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.

Immunofluorescence

Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunofluorescent analysis of CD210a staining in HUVEC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 ??C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot

Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.
Western blot analysis of CD210a expression in HUVEC (A); HEK293T (B) whole cell lysates.

Requested From: United States
Date Requested: 11/15/2018

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