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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154104-100 100 µg (1 mg/ml) $425 
IL-33 Antibody - Anti-Human IL-33 Antibody - Western Blot. Western blot of HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kD in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal IL‑33 Antibody (HRP, IHC, WB) LS‑C154104

Polyclonal IL‑33 Antibody (HRP, IHC, WB) LS‑C154104

Antibody:
Rabbit Polyclonal to Human IL-33
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$425 
LS-C154104-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human IL-33
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
IL-33 antibody LS-C154104 is an HRP-conjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA, IHC and WB.
Target
Human IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Specificity
This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 18 kD in size corresponding to mature human IL-32a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

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Requested From: United States
Date Requested: 4/6/2020