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Catalog Number Size Price
LS-C154104-100 100 µg (1 mg/ml) $552 
IL-33 Antibody - Anti-Human IL-33 Antibody - Western Blot. Western blot of HRP conjugated anti-Human IL-33 antibody shows detection of a band ~18 kD in size corresponding to recombinant human IL-33. The identity of the higher molecular weight band is unknown. Molecular weight markers are shown (left). After transfer, the membrane was blocked with 3% BSA in TBS followed by reaction with antibody at a 1:5000 dilution for 30 min at room temperature. Detection occurred using TMB substrate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Polyclonal Rabbit anti‑Human IL‑33 Antibody (HRP, IHC, WB) LS‑C154104

Polyclonal Rabbit anti‑Human IL‑33 Antibody (HRP, IHC, WB) LS‑C154104

Antibody:
IL-33 Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$552
LS-C154104-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
IL-33 Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
IL-33 antibody LS-C154104 is an HRP-conjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA, IHC and WB.
Target
Human IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Antiserum
Modifications
Unmodified
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Specificity
This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Applications
  • IHC (1:500 - 1:2500)
  • Western blot (1:1000 - 1:5000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 18 kD in size corresponding to mature human IL-32a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024