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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C154084-100 100 µg (1 mg/ml) $425 
IL-33 Antibody - Anti-Human IL-33 Antibody - Western Blot. Western blot of anti-Human IL-33 antibody shows detection of a band ~18 kD in size corresponding to recombinant human IL-33 (lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (LS-C60865) secondary antibody diluted 1:40000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reaction with FemtoMax chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).

Polyclonal Rabbit anti‑Human IL‑33 Antibody (Lyophilized, WB) LS‑C154084

Polyclonal Rabbit anti‑Human IL‑33 Antibody (Lyophilized, WB) LS‑C154084

Antibody:
Rabbit Polyclonal to Human IL-33
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized
Price
Catalog Number
$425 
LS-C154084-100
100 µg
1 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human IL-33
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Lyophilized

Specifications

Description
IL-33 antibody LS-C154084 is an unconjugated rabbit polyclonal antibody to human IL-33. Validated for ELISA and WB.
Target
Human IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Lyophilized
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:20000 - 1:100000)
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-33 protein.
Specificity
This antibody will recognize both native and recombinant human IL-33 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Usage
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 18 kD in size corresponding to mature human IL-32a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About IL-33
O95760 NM_033439 NP_254274.1

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Requested From: United States
Date Requested: 6/6/2020