Human IL-1B / IL-1 Beta
Human, Dog, Primate
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:100 - 1:200)
- IHC - Frozen (1:100 - 1:200)
- Western blot (1:100 - 1:200)
- Immunoprecipitation (1:400 - 1:800)
- ELISA (1:500 - 1:2000)
- Radioimmunoassay (1:5000 - 1:10000)
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Recombinant human IL1B / IL1 Beta.
Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection.
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.
This antibody carries the LSBio 100% Guarantee.