(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Full length recombinant human IL 1 beta protein
Recognizes human IL-1 beta. (Does not recognize mouse IL-1 beta (human IL-1 alpha or a panel of other human cytokines.
Neutralization: This antibody has been used at a 100-fold molar excess to neutralize 95% of the biological activity of human IL-1 . Immunohistochemistry: This antibody has been used at 1-10 µg/ml to stain human hIL- 1 expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4°C, plus 0.1% saponin 20 minutes RT. Immunoprecipitation: This antibody has been used to immunoprecipitate >90% hIL-1 in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). ELISA: This antibody has been used as a solution phase antibody at 2 µg/ml in a two site ELISA with biotin conjugated IL-1beta antibody. Typically the ELISA has a working range of 0-500 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of hIL-1(in human serum) is typically >85% in the ELISA
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.