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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL-1B / IL-1 Beta Antibody (clone AS10) LS-C26495

IL-1 Beta antibody LS-C26495 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA, IHC, IP and Neut. Cited in 1 publication.
500 µg (1 mg/ml)
IL-1 Beta antibody LS-C26495 is an unconjugated mouse monoclonal antibody to human IL-1 Beta (IL-1B). Validated for ELISA, IHC, IP and Neut. Cited in 1 publication.
Human IL-1B / IL-1 Beta
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated. Also available conjugated with Biotin.
Protein A/G purified
  • IHC
  • Immunoprecipitation
  • Neutralization
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Full length recombinant human IL 1 beta protein
Recognizes human IL-1 beta. (Does not recognize mouse IL-1 beta (human IL-1 alpha or a panel of other human cytokines.
Neutralization: This antibody has been used at a 100-fold molar excess to neutralize 95% of the biological activity of human IL-1 . Immunohistochemistry: This antibody has been used at 1-10 µg/ml to stain human hIL- 1 expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4°C, plus 0.1% saponin 20 minutes RT. Immunoprecipitation: This antibody has been used to immunoprecipitate >90% hIL-1 in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). ELISA: This antibody has been used as a solution phase antibody at 2 µg/ml in a two site ELISA with biotin conjugated IL-1beta antibody. Typically the ELISA has a working range of 0-500 pg/ml and a sensitivity of 5 pg/ml with the appropriate standard. Recommended sample volumes are 25-200 µl. Recovery of hIL-1(in human serum) is typically >85% in the ELISA
PBS, pH 7.4
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles.
For research use only.
About IL-1B / IL-1 Beta
P01584 NM_000576 NP_000567.1

Popular IL-1B / IL-1 Beta Products

IHC of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-IL1B mouse monoclonal antibody.
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Anti-Human IL-1B Antibody - Western Blot. IL-1B was used at a 1:200 dilution incubated 1 h at room temperature to detect dog IL-1B by Western blot. Lane 1 and 2 were loaded with 2.5 ug and 500 ng of dog IL-1B respectively. The molecular weight of the detected band is estimated by comparison to molecular weight markers (not shown). Detection occurred using a 1:3000 dilution of IRDye800 conjugated Donkey anti-Rabbit IgG (code # for 1h at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.
Species: Human, Dog, Primate
Applications: IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, ELISA, Radioimmunoassay
Species: Sheep
Applications: Western blot, ELISA

Publications (1)

Autophagy Mediates Interleukin-1ß Secretion in Human Neutrophils. Iula L, Keitelman IA, Sabbione F, Fuentes F, Guzman M, Galletti JG, Gerber PP, Ostrowski M, Geffner JR, Jancic CC, Trevani AS. Frontiers in immunology. 2018 February;9:269. (Human) [Full Text Article] [PubMed:29515581] [PMC:PMC5825906]

Customer Reviews (0)

Requested From: United States
Date Requested: 2/21/2019
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