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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IKBKB / IKK2 / IKK Beta Antibody (clone 10A9B6) LS‑C555
IKK Beta antibody LS-C555 is an unconjugated mouse monoclonal antibody to IKK Beta (IKBKB / IKK2) from human and mouse. Validated for IP and WB.
Catalog
Size
Price
LS-C555-100
100 µg (0.5 mg/ml)
$335

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Applications: IHC, Western blot, ELISA

Product Description

IKK Beta antibody LS-C555 is an unconjugated mouse monoclonal antibody to IKK Beta (IKBKB / IKK2) from human and mouse. Validated for IP and WB.

Specifications

Target
Human IKBKB / IKK2 / IKK Beta
Synonyms
IKBKB, Ikk 2, IKK-B, IKK2, IKKbeta, NFKBIKB, I-kappa-B kinase 2, IKKB, I-kappa-B-kinase beta, Ikb kinase-beta, IKK Beta, IKK-beta
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [10A9B6]
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • Western blot (2 - 5 µg/ml)
  • Immunoprecipitation (2 - 5 µg/10E6 cells)
Failed Applications
  • IHC - Paraffin
Immunogen
Full length human IKKb/IKK2 recombinant protein.
Specificity
An approx. 87 kD band should be observed.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About IKBKB / IKK2 / IKK Beta
Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. O14920 AF080158 AAD08997.1


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Images

Western blot

Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.
Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.

Immunoprecipitation

antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.
antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.

Western blot

Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.
Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.

Immunoprecipitation

antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.
antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.

Western blot

Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.
Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.

Immunoprecipitation

antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.
antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.

Western blot

Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.
Western blot of IKKb in Daudi cell lysate using antibody at5 ug/ml.

Immunoprecipitation

antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.
antibody was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using antibody. IKKb was detected as a protein of ~87 kD.

Requested From: United States
Date Requested: 10/19/2018

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