Menu
Products
Services
Research Areas
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Services
Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, froforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-B16263-50 50 µg (200 µg/ml) $425 
WT1 / Wilms Tumor 1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
WT1 / Wilms Tumor 1 Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
WT1 / Wilms Tumor 1 Antibody - SDS-PAGE Analysis of Purified Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857). Confirmation of Purity and Integrity of Antibody.
WT1 / Wilms Tumor 1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
WT1 / Wilms Tumor 1 Antibody - Analysis of Protein Array containing more than 19,000 full-length human proteins using Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.  A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
WT1 / Wilms Tumor 1 Antibody - SDS-PAGE Analysis of Purified Wilm’s Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857). Confirmation of Purity and Integrity of Antibody.
1 of 3
2 of 3
3 of 3

IHCPlus™ WT1 / Wilms Tumor 1 Antibody Recombinant Monoclonal IHC LS‑B16263

IHCPlus™ WT1 / Wilms Tumor 1 Antibody Recombinant Monoclonal IHC LS‑B16263

Note: This antibody replaces LS-C751419
Antibody:
Mouse Recombinant Monoclonal to Human WT1 / Wilms Tumor 1
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B16263-50
50 µg
200 µg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Recombinant Monoclonal to Human WT1 / Wilms Tumor 1
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
Wilms Tumor 1 antibody LS-B16263 is an unconjugated mouse recombinant monoclonal antibody to human Wilms Tumor 1 (WT1 ). Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human WT1 / Wilms Tumor 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Recombinant Monoclonal
Clone
rWT1/857
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified. Also available Azide-free, Carrier-free.
Applications
  • IHC - Paraffin (1:50)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant full-length human WT1 protein. Antigen Molecular Weight: 47-55 kDa
Specificity
Recognizes a 47-55 kDa -tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm's tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm's tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm's tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Usage
Immunohistology (Formalin-fixed - 0.5-1 ug/ml for 30 minutes at RT), Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.) Optimal dilution for a specific application should be determined.
Presentation
1 mM PBS, 0.05% BSA, 0.05% Sodium Azide
Storage
Store at 2°C to 8°C for up to 2 years.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WT1 / Wilms Tumor 1
P19544 NM_024426 NP_077744.3

Validation

WT1 / Wilms Tumor 1 Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Publications (0)

Customer Reviews (0)


Requested From: United States
Date Requested: 12/7/2019