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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B5896-100 100 µl (0.5 mg/ml) $485 
WNT3A Antibody - Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WNT3A Antibody - WNT3A antibody Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
WNT3A Antibody - Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WNT3A Antibody - WNT3A antibody Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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IHCPlus™ WNT3A Antibody aa227‑276 Polyclonal IHC,WB LS‑B5896

IHCPlus™ WNT3A Antibody aa227‑276 Polyclonal IHC,WB LS‑B5896

Note: This antibody replaces LS-C134885
Antibody:
Rabbit Polyclonal to Mouse WNT3A
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B5896-100
100 µl
0.5 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Mouse WNT3A
Application:
IHC, IHC-P, WB
Reactivity:
Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
WNT3A antibody LS-B5896 is an unconjugated rabbit polyclonal antibody to WNT3A from human, mouse, rat and other species. Validated for IHC and WB.
Target
Mouse WNT3A
Host
Rabbit
Reactivity
Mouse, Human, Monkey, Rat, Bovine, Pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (1 µg/ml)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide located between aa227-276 of mouse Wnt3a (P27467, NP_033548). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Bovine, Pig (100%); Dog, Horse, Guinea pig (92%); Gorilla, Rabbit, Platypus (85%).
Blocking Peptide
LS-E21525 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5896. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa227-276
Specificity
Mouse WNT3A
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About WNT3A
P56704 NM_033131 NP_149122.1

Validation

WNT3A Antibody - Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-WNT3A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

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Requested From: United States
Date Requested: 11/12/2019