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IHCPlus™ VHL / Von Hippel Lindau Antibody aa167‑179 Polyclonal ELISA,IHC,Peptide‑ELISA,WB LS‑B10235

Catalog Number Size Price
LS-B10235-50 50 µg (0.5 mg/ml) $425 
Anti-VHL / Von Hippel Lindau antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
VHL / Von Hippel Lindau antibody (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
VHL antibody (0.01 ug/ml) staining of Human Ovary lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
VHL / Von Hippel Lindau antibody (1.5ug/ml) as the reporter with EB002014 as the capture rabbit antibody (2.5ug/ml).
Anti-VHL / Von Hippel Lindau antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
VHL / Von Hippel Lindau antibody (0.01µg/ml) staining of Human Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
VHL antibody (0.01 ug/ml) staining of Human Ovary lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
VHL / Von Hippel Lindau antibody (1.5ug/ml) as the reporter with EB002014 as the capture rabbit antibody (2.5ug/ml).
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IHCPlus™ VHL / Von Hippel Lindau Antibody aa167‑179 Polyclonal ELISA,IHC,Peptide‑ELISA,WB LS‑B10235

Note: This antibody replaces LS-C34681
Antibody:
Goat Polyclonal to Human VHL / Von Hippel Lindau
Application:
IHC, IHC-P, WB, ELISA, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Dog, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B10235-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human VHL / Von Hippel Lindau
Application:
IHC, IHC-P, WB, ELISA, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Dog, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Von Hippel Lindau antibody LS-B10235 is an unconjugated goat polyclonal antibody to Von Hippel Lindau (VHL ) from human, mouse, rat and other species. Validated for ELISA, IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human VHL / Von Hippel Lindau
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.01 - 0.03 µg/ml)
  • ELISA (1.5 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-RSLVKPENYRRLD, from the internal region of the protein sequence according to NP_000542.1NP_937799.1.
Blocking Peptide
LS-E26135 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B10235. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa167-179
Specificity
Human VHL. This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 17 kDa band observed in Human Ovary and Testis lysates and in lysates of all cell lines tested (calculated MW of 19.7 kDa according to NP_937799.1). Recommended concentration: 0.01-0.03 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VHL / Von Hippel Lindau
P40337 NM_000551 NP_000542.1

Validation

Anti-VHL / Von Hippel Lindau antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-VHL / Von Hippel Lindau antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-VHL / Von Hippel Lindau antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Requested From: United States
Date Requested: 9/22/2019