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Catalog Number Size Price
LS-B1912-50 50 µg (0.5 mg/ml) $460 
VCP Antibody - Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
VCP Antibody - Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI
VCP Antibody - Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
VCP Antibody - Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
VCP Antibody - Overnight nocodazole treated Hela cells stained with purified mouse monoclonal antibody against p97/VCP (clone 4G9), followed by Cy3 conjugated goat anti-mouse IgG and DAPI
VCP Antibody - Western blot of extracts from HeLa cells (Lane 1), Jurkat cells (Lane 2), MCF7 cells (Lane 3), or mouse splenocytes (Lane 4), using anti-p97/VCP, clone 4G9
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IHC‑plus™ Monoclonal Mouse anti‑Human VCP Antibody (clone 4G9, IHC, IF, WB) LS‑B1912

IHC‑plus™ Monoclonal Mouse anti‑Human VCP Antibody (clone 4G9, IHC, IF, WB) LS‑B1912

Note: This antibody replaces LS-C40951
Antibody:
VCP Mouse anti-Human Monoclonal (4G9) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B1912-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
VCP Mouse anti-Human Monoclonal (4G9) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, IP
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
VCP antibody LS-B1912 is an unconjugated mouse monoclonal antibody to human VCP. Validated for ICC, IF, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues. Cited in 3 publications.
Target
Human VCP
Synonyms
VCP | 15S Mg(2+)-ATPase p97 subunit | ALS14 | TER ATPase | TERA | Valosin containing protein | Yeast Cdc48p homolog | Valosin-containing protein | IBMPFD | p97
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
4G9
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant human VCP.
Specificity
Recombinant human valosin containing protein
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • ICC
  • Immunofluorescence (1 - 4 µg/ml)
  • Western blot
  • Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B1912 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1912 was determined to be 5 ug/ml.
Presentation
Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VCP
P55072 NM_007126 NP_009057.1

Validation

VCP Antibody - Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-VCP antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

IHC-plus™ VCP Antibody (clone 4G9) for IHC, ICC, IF/Immunofluorescence, WB/Western, IP LS-B1912 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (4)

Publications (3)

Selenoprotein K binds multiprotein complexes and is involved in the regulation of endoplasmic reticulum homeostasis. Shchedrina VA, Everley RA, Zhang Y, Gygi SP, Hatfield DL, Gladyshev VN. The Journal of biological chemistry. 2011 286:42937-48.

Altered intracellular localization and valosin-containing protein (p97 VCP) interaction underlie ATP7A-related distal motor neuropathy. Yi L, Donsante A, Kennerson ML, Mercer JF, Garbern JY, Kaler SG. Human molecular genetics. 2012 21:1794-807.

Regulation of subtilase cytotoxin-induced cell death by an RNA-dependent protein kinase-like endoplasmic reticulum kinase-dependent proteasome pathway in HeLa cells. Yahiro K, Tsutsuki H, Ogura K, Nagasawa S, Moss J, Noda M. Infection and immunity. 2012 80:1803-14.

Customer Reviews (0)


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Requested From: United States
Date Requested: 4/19/2024