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IHCPlus™ UBC / Ubiquitin C Antibody aa1‑32 Polyclonal IHC,WB LS‑B3386

Catalog Number Size Price
LS-B3386-200 200 µl $425 
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of anti-Ubiquitin antibody in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Western blot of anti-Ubiquitin antibody in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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IHCPlus™ UBC / Ubiquitin C Antibody aa1‑32 Polyclonal IHC,WB LS‑B3386

Note: This antibody replaces LS-C98776
Antibody:
Rabbit Polyclonal to Human UBC / Ubiquitin C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3386-200
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human UBC / Ubiquitin C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Ubiquitin C antibody LS-B3386 is an unconjugated rabbit polyclonal antibody to human Ubiquitin C (UBC ). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human UBC / Ubiquitin C
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Hamster, Pig, Rabbit, Chicken, Xenopus, Drosophila, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1551 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B3386. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-32
Specificity
This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.
Usage
Immunohistochemistry: LS-B3386 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3386 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBC / Ubiquitin C
P0CG48 M26880 AAA36789.1

Validation

Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC Validated
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Publications (1)

Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini. Ouyang H, Ali YO, Ravichandran M, Dong A, Qiu W, MacKenzie F, Dhe-Paganon S, Arrowsmith CH, Zhai RG. The Journal of biological chemistry. 2012 287:2317-27.

Reviews (0)

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Requested From: United States
Date Requested: 9/21/2019