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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B3386-200 200 µl $425 
UBC / Ubiquitin C Antibody - Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
UBC / Ubiquitin C Antibody - Western blot of anti-Ubiquitin antibody in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
UBC / Ubiquitin C Antibody - Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
UBC / Ubiquitin C Antibody - Western blot of anti-Ubiquitin antibody in HeLa cell lysate. Ubiquitin (Arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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IHCPlus™ Polyclonal Rabbit anti‑Human UBC / Ubiquitin C Antibody (aa1‑32, IHC, WB) LS‑B3386

IHCPlus™ Polyclonal Rabbit anti‑Human UBC / Ubiquitin C Antibody (aa1‑32, IHC, WB) LS‑B3386

Note: This antibody replaces LS-C98776
Antibody:
Rabbit Polyclonal to Human UBC / Ubiquitin C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B3386-200
200 µl
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human UBC / Ubiquitin C
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Ubiquitin C antibody LS-B3386 is an unconjugated rabbit polyclonal antibody to human Ubiquitin C (UBC) (aa1-32). Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human UBC / Ubiquitin C
Synonyms
UBC | HUBCEP52 | L40 | HMG20 | RPL40 | Ubiquitin | Polyubiquitin-C | CEP52 | Ubiquitin C
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Hamster, Pig, Rabbit, Chicken, Xenopus, Drosophila, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E1551 - Lyophilized - 500 µg - $360.00
Immunizing peptide used to generate LS-B3386. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa1-32
Specificity
This Ubiquitin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-32 amino acids from the N-terminal region of human Ubiquitin.
Usage
Immunohistochemistry: LS-B3386 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3386 was determined to be 10 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UBC / Ubiquitin C
P0CG48 M26880 AAA36789.1

Validation

UBC / Ubiquitin C Antibody - Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-UBC / Ubiquitin C antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ UBC / Ubiquitin C Antibody (aa1-32) for IHC, WB/Western LS-B3386 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Protein aggregates are recruited to aggresome by histone deacetylase 6 via unanchored ubiquitin C termini. Ouyang H, Ali YO, Ravichandran M, Dong A, Qiu W, MacKenzie F, Dhe-Paganon S, Arrowsmith CH, Zhai RG. The Journal of biological chemistry. 2012 287:2317-27.

Customer Reviews (0)


Requested From: United States
Date Requested: 7/9/2020