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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-B10184-0.05 0.05 mg (1 mg/ml) $425 
TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TRPV4 Antibody - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 µg/ml.
TRPV4 Antibody - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 µg/mL.
TRPV4 Antibody - Western blot analysis of TRPV4 in human testis tissue lysate with TRPV4 antibody at 1 ug/ml.
TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
TRPV4 Antibody - Immunofluorescence of TRPV4 in human kidney tissue with TRPV4 antibody at 5 µg/ml.
TRPV4 Antibody - Immunofluorescence of TRPV4 in human lung tissue with TRPV4 antibody at 20 µg/mL.
TRPV4 Antibody - Western blot analysis of TRPV4 in human testis tissue lysate with TRPV4 antibody at 1 ug/ml.
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IHC‑plus™ Polyclonal Rabbit anti‑Human TRPV4 Antibody (IHC, IF, WB) LS‑B10184

IHC‑plus™ Polyclonal Rabbit anti‑Human TRPV4 Antibody (IHC, IF, WB) LS‑B10184

Note: This antibody replaces LS-C201191
Antibody:
TRPV4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425
LS-B10184-0.05
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
TRPV4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
TRPV4 antibody LS-B10184 is an unconjugated rabbit polyclonal antibody to human TRPV4. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human TRPV4
Synonyms
TRPV4 | CMT2C | HMSN2C | OTRPC4 | SSQTL1 | VR-OAC | VRL-2 | Osm-9-like TRP channel 4 | SPSMA | TRP12 | VRL2 | VROAC | SMAL
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
An 18 AA peptide near the center of human TRPV4. The immunogen is located within aa 380-430 of TRPV4.
Blocking Peptide
LS-E45911 - Liquid - 0.05 mg 200 µg/ml - $145.00
Immunizing peptide used to generate LS-B10184. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Specificity
TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (20 µg/ml)
  • Western blot (1 - 2 µg/ml)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 ug/ml. Antibody can also be used for Immunohistochemistry starting at 2 ug/mL. For immunofluorescence start at 5 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 96 kDa Observed: 100 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About TRPV4
Q9HBA0 NM_021625 NP_067638.3

Validation

TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-TRPV4 antibody IHC staining of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

TRPV4 Antibody - Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-TRPV4 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ TRPV4 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B10184 has an LSBio Rating of
Laboratory Validation Score (4)

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Requested From: United States
Date Requested: 10/20/2020